Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Starch Column
نویسندگان
چکیده
منابع مشابه
Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase.
alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-...
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BACKGROUND Starch is a very abundant and renewable carbohydrate and is an important feedstock for industrial applications. The conventional starch liquefaction and saccharification processes are energy-intensive, complicated, and not environmentally friendly. Raw starch-digesting glucoamylases are capable of directly hydrolyzing raw starch to glucose at low temperatures, which significantly sim...
متن کاملThermostable, Raw-Starch-Digesting Amylase from Bacillus stearothermophilus.
An endospore-forming thermophilic bacterium, which produced amylase and was identified as Bacillus stearothermophilus, was isolated from soil. The amylase had an optimum temperature of 70 degrees C and strongly degraded wheat starch granules (93%) and potato starch granules (80%) at 60 degrees C.
متن کاملRaw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
BACKGROUND Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing (CBP) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol. RESULTS The Aspergillus tubing...
متن کاملA Simplified Starch Determination for Raw Sugars
The conventional starch determination (employing precipitation, filtration, re-dissolution, colour development) is compared with a simplified direct colorimetric procedure, which is readily automated. The reproducibility is better than 3%, with a correlation coefficient of 0,87 between the two methods.
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1996
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.60.1717